Reply to “Structural requirements for RNA degradation by HIV-1 reverse transcriptase”
نویسندگان
چکیده
منابع مشابه
HIV-1 reverse transcriptase connection subdomain mutations reduce template RNA degradation and enhance AZT excision.
We previously proposed that mutations in the connection subdomain (cn) of HIV-1 reverse transcriptase increase AZT resistance by altering the balance between nucleotide excision and template RNA degradation. To test the predictions of this model, we analyzed the effects of previously identified cn mutations in combination with thymidine analog mutations (D67N, K70R, T215Y, and K219Q) on in vitr...
متن کاملConformational changes in HIV-1 reverse transcriptase induced by nonnucleoside reverse transcriptase inhibitor binding.
Nonnucleoside reverse transcriptase inhibitors (NNRTI) are a group of small hydrophobic compounds with diverse structures that specifically inhibit HIV-1 reverse transcriptase (RT). NNRTIs interact with HIV-1 RT by binding to a single site on the p66 subunit of the p66/p51 heterodimeric enzyme, termed the NNRTI-binding pocket (NNRTI-BP). This binding interaction results in both short-range and ...
متن کاملPotent Inhibition of HIV-1 Reverse Transcriptase and Replication by Nonpseudoknot, “UCAA-motif” RNA Aptamers
RNA aptamers that bind the reverse transcriptase (RT) of human immunodeficiency virus (HIV) compete with nucleic acid primer/template for access to RT, inhibit RT enzymatic activity in vitro, and suppress viral replication when expressed in human cells. Numerous pseudoknot aptamers have been identified by sequence analysis, but relatively few have been confirmed experimentally. In this work, a ...
متن کاملHIV-1 CRF08_BC mutants resistant to reverse transcriptase inhibitors
Human immunodeficiency virus type (HIV)-1 circulating recombinant form 08_BC (CRF08_BC), carrying recombinant reverse transcriptase (RT) gene from subtype B and C, has recently become highly prevalent in Southern China. As the number of patients infected by CRF08_BC increases, it is important to characterize the drug resistance mutations of CRF08_BC, especially against widely used antiretrovira...
متن کاملStructural Maturation of HIV-1 Reverse Transcriptase—A Metamorphic Solution to Genomic Instability
Human immunodeficiency virus 1 (HIV-1) reverse transcriptase (RT)-a critical enzyme of the viral life cycle-undergoes a complex maturation process, required so that a pair of p66 precursor proteins can develop conformationally along different pathways, one evolving to form active polymerase and ribonuclease H (RH) domains, while the second forms a non-functional polymerase and a proteolyzed RH ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Structural & Molecular Biology
سال: 2013
ISSN: 1545-9993,1545-9985
DOI: 10.1038/nsmb.2726